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A versatile ionic strength sensitive tag from a human GM CSF derived linear epitope
(Elsevier, 2013-06)
A 7-mer hGM-CSF-derived linear epitope (APARSPS) is herein described as a novel and small tag taking into account its particular binding affinity in native conditions that could be easily modified by increasing or lowering ...
Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli
(Elsevier Science, 2021-05)
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as ...
Redox-tagged peptide for capacitive diagnostic assays
(2014)
Early detection assays play a key role in the successful treatment of most diseases. Redox capacitive biosensors were recently introduced as a potential electroanalytical assay platform for point-of-care applications but ...
Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids
(American Society for Microbiology, 2013-09)
Addition of affinity tags to bacteriophage particles facilitates a variety of applications, including vaccine construction and diagnosis of bacterial infections. Addition of tags to phage capsids is desirable, as modification ...
Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine
(Wiley, 2014-09)
Fusion of peptide-based tags to recombinant proteins is currently one of the most used tools for protein production. Also, immobilized metal ion affinity chromatography (IMAC) has a huge application in protein purification, ...
Optimization of the isotope-coded affinity tag-labeling procedure for quantitative proteome analysis
(Academic Press IncSan DiegoEUA, 2001)
Interaction of histidine-tagged human proinsulin with immobilized nickel ion: Effect of chelating ligand and thermodynamics analysis
(Elsevier Science BvAmsterdamHolanda, 2010)